That’s great for throughput, but very challenging for your sample sheets.
The number of samples in a single sequencing run has become so large it is sometimes necessary to put both index and barcode sequences on samples. Track Both Barcode and Index Sequences at the Same Time This must occur within a single batch of pooled samples, and also between pools that are aggregated and sequenced together. Summary: A good LIMS must assess for indexes that clash between samples. These super-pools present the biggest potential for error because, as is often the case, multiple people prepare them and don’t always accurately communicate which indexes or barcodes were used. It is now a vital requirement that any LIMS assess for index clashes both within a batch of samples that is pooled, and between any pools that are aggregated, making those complex super-pools. But now, labs are sequencing hundreds of samples at a time. An absolute requirement for any LIMS is that the system automatically reviews all samples in a sequencing pool to ensure there are no clashing index sequences.Īt one time, avoiding these clashes was simpler because a pool consisted of just a handful of samples. Speaking of cold-sweat-inducing nightmares, there is nothing worse than realizing you’ve had an index clash… and realizing it only after your run is complete and you’re reviewing your data. Check for Index-Clashing Both Within and Between Pools You should never have to manually enter a sequence during sample tracking the risk of error is simply too great. Implement a LIMS that has index sequences already pre-loaded.
SAMPLE MANAGER LIMS USER MANUAL MANUAL
Summary: By having the fewest possible manual steps in your process, you’ll prevent errors and maintain sample integrity. There should be simple methods for you to quickly choose the pre-loaded index plate, and the well on the plate, that was used for each sample in your batch. With a good LIMS, you should also be able to easily select the correct index for each sample you prepare. This ensures that no errors arise due to manually entering sequences, or copy and pasting them, each time you prepare libraries. Then, when you create new libraries, you can select from the index sequences already entered and available. A good LIMS should have all the index sequences you use pre-loaded into the system in your lab. To reduce or eliminate errors, cutting down on manual data entry is paramount. Accurately Track Index Sequences During Sequencing Library Preparation By using a Laboratory Information Management System (LIMS) for automated sample management in your lab, you can track your samples during the preparation of sequencing libraries, and prevent issues before ever using your precious sequencing reagents.īut what’s the right LIMS for your lab? What system characteristics are critical to getting rid of sample management frustrations and maintaining tight process control? To ensure you avoid unnecessary mistakes, always consider how a LIMS handles the following four situations: 1. Thankfully, there are easy ways to avoid these frustrating and costly errors. If you’re testing clinical samples, and your clinical sample management is compromised, the situation carries even greater consequences: delayed clinical results, which negatively impact patients. You’re forced to re-prepare and re-sequence your libraries, a time-consuming headache that can also be extremely expensive. These sample and data management mistakes are easy to make, and can cause you to break into a cold sweat. Have you ever accidentally copied the wrong index sequence onto your next-generation sequencing (NGS) sample tracking sheet, unsure which well you used from an index plate? How about mistakenly including two samples in a pool that both have the same index or barcode sequence? Worse yet, maybe you didn’t recognize you made one of these mistakes, and your data and sample integrity were compromised for the entire sequencing run…
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